GOT1 Sheep Polyclonal Antibody

CAT#: R1087PS

GOT1 sheep polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western blot: 1/500-1/3,000.
  ELISA: 1/5,000-1/25,000.
  This antibody has been assayed against 1.0 µg of aspartate transaminase [Porcine heart] in a standard sandwich ELISA using peroxidase conjugated affinity purified goat anti-sheep IgG [H&L] (R1364HRP) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/7,000 to 1/26,000 of the reconstitution concentration is suggested.
• Reactivities: Porcine
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: Aspartate aminotransferase / GOT1 from porcine heart
• Specificity: This antibody detects porcine GOT1.
  Immunoelectrophoresis gives a single precipitin arc against anti-sheep serum as well as purified and partially purified aspartate transaminase / GOT1 [porcine heart].
• Formulation: 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
  State: Purified
  State: Lyophilized purified Ig fraction
  Preservative: 0.01% Sodium azide
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P00503
• Background: Aspartate aminotransferase (Glutamate oxaloacetate transaminase / GOT1) is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene.
  Serum GOT (with SGPT) levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.
• Synonyms: Aspartate aminotransferase, Transaminase A
• Note: Aspartate aminotransferase (AST) is also referred to as glutamic oxaloacetic transaminase (GOT).
  Conjugates available. Please ask for details.