Dextranase Rabbit Polyclonal Antibody

CAT#: R1077HRPS

Dextranase rabbit polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/5000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5000-1/20000.
  This product has been assayed against 1.0 µg of dextranase [Penicillium species] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/3,000 of the reconstitution concentration is suggested.
• Reactivities: Penicillium
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Dextranase from Penicillium species
• Specificity: This antibody detects dextranase [Penicillium species]. Cross reactivity against dextranase from other sources is unknown.
  Immunoelectrophoresis gives a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified dextranase [Penicillium species].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunogobulin and protease free)
  Preservative: 0.01% Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P48845
• Background: Dextranase is a transferase enzyme that catalyzes the synthesis of dextran from dextrin.
• Synonyms: DEX
• Note: Biotin conjugated antibody and unconjugated, purified antibody available. Please ask for details.