lacZ Rabbit Polyclonal Antibody

CAT#: R1064FS

lacZ rabbit polyclonal antibody, FITC, Purified

Product Images

Western blotting using fluorescein conjugated anti-Beta-galactosidase tag antibody shows a band at ~117 kDa (lanes 1-3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate. Proteins were resolved on a 4-20% Tris-glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using blocking buffer for bluorescent Western blotting. The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase. [R1064F] diluted to 1/10,000. Reaction occurred for 2 hours at RT. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc® imaging system. Other detection systems will yield similar results.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: This product is designed for fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended Dilutions:
  Western blot: 1/10,000.
  IF microscopy: 1/500-1/2,500.
• Reactivities: Escherichia coli
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Beta-galactosidase from E.coli
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum as well as purified and partially purified beta galactosidase (E.coli).
• Formulation: 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2
  Label: FITC
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Sodium azide
  Label: Fluorescein isothiocyanate (molecular weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 3.5 moles FITC per mole of IgG
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: FITC
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P00722
• Synonyms: Beta-Gal tag, lacZ tag, b0344, JW0335, Beta-Gal Fusion Protein, Lactase