Alpha-amylase Rabbit Polyclonal Antibody

CAT#: R1062HRPS

Alpha-amylase rabbit polyclonal antibody, HRP, Purified


USD 740.00

3 Days*

Size
    • 100 ug

Product Images

Specifications

Product Data
Applications ELISA, IP, WB
Recommended Dilution

Western blot: 1/500-1/5,000.
Immunoprecipitation: 1/100.
ELISA: 1/5,000-1/20,000.
This antibody has been assayed against 1.0 µg of Alpha-amylase in a standard  capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/10,000 of the reconstitution concentration is suggested.

Reactivities Bacillus amyloliquefacies
Host Rabbit
Clonality Polyclonal
Immunogen Alpha-amylase from Bacillus amyloliquefaciens
Specificity This antibody detects Alpha-amylase (Bacillus amyloliquefaciens). Cross reactivity against Alpha-amylase from other tissues and species may occur but have not been specifically determined.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum as well aspurified and partially purified Alpha-amylase from Bacillus amyloliquefaciens.
Formulation 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Label: HRP
State: Purified
State: Lyophilized purified Ig fraction
Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
Label: Horseradish peroxidase
Concentration lot specific
Purification Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation HRP
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Amylase catalyses the hydrolysis of internal 1,4 glucan links in large linear polymers at internal bonds. The enzyme is present in all living organisms with variable enzymatic activity.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.