Galectin 1 (LGALS1) Rabbit Polyclonal Antibody

CAT#: PP1223B2

Galectin 1 (LGALS1) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human Galectin-1 by direct ELISA (using 100 µl/well antibody solution), this antibody can be used at a concentration of ~1.0 µg/ml. This Biotin conjugated antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human Galectin-1.
  Sandwich ELISA: To detect Human Galectin-1 by Sandwich ELISA (using 100 µl/well antibody solution), this antibody can be used at a concentration of 0.25-1.0 µg/ml. This Biotin conjugated antibody, in conjunction with Purified anti-Human Galectin-1 antibody (PP1223P) as Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Galectin-1.
  Western blot: To detect Human Galectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: E.coli derived recombinant Human Galectin-1.
• Specificity: Reacts with Galectin-1.
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS solution containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C.
  Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: galectin 1
• Database Link:
  Entrez Gene 3956 Human
  UniProt ID P09382
• Background: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 1 may act as an autocrine negative growth factor that regulates cell proliferation. It also may regulate cell apoptosis and cell differentiation. It binds beta-galactoside, CD45, CD3 and CD4. Galectin 1 inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. It is found in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney, placenta and hematopoietic cells.
• Synonyms: LGALS1, Galectin1, Lectin galactoside-binding soluble 1, Beta-galactoside-binding lectin L-14-I, Lactose-binding lectin 1, S-Lac lectin 1, Galaptin, 14 kDa lectin, HPL, HBL, MAPK-activating protein PM12