Il3 Goat Polyclonal Antibody

CAT#: PP1127P2

Il3 goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-3beta (100 ng/ml), a concentration of 2.7-4.0 µg/ml of this antibody is required.
  ELISA: To detect rat IL-3 beta by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat IL-3 beta.
  Western Blot: To detect rat IL-3 beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-3 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant rIL-3 beta.
• Specificity: Rat Interleukin-3 beta
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody prior to reconstitution at -20°C.
  Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: interleukin 3
• Database Link:
  Entrez Gene 24495 Rat
  UniProt ID P97688
• Background: IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells.
• Synonyms: IL-3 beta, IL3B
• Note: Centrifuge vial prior to opening!