Egf Goat Polyclonal Antibody

CAT#: PP1126B2

Egf goat polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: ELISA:
  (Direct): To detect mEGF by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF.
  (Sandwich): To detect mEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine EGF (PP1126P) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF.
  Western Blot: To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant mEGF.
• Specificity: Murine EGF
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: epidermal growth factor
• Database Link:
  Entrez Gene 13645 Mouse
  UniProt ID P01132
• Background: Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein.
  Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.
• Synonyms: Urogastrone, Epidermal growth factor, URG, HOMG4
• Note: Centrifuge vial prior to opening!