TNF alpha (TNF) Rabbit Polyclonal Antibody

CAT#: PP1071P2

TNF alpha (TNF) rabbit polyclonal antibody, Aff - Purified

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Immunohistochemistry: This TNF-alpha antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.5 ug/ml-1.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

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Specifications

Product Data

• Applications: ELISA, FN, IF, IHC, WB
• Recommended Dilution: Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity of hTNF-alpha (0.25 ng/ml), a concentration of 0.08-0.010 µg/ml of this antibody is required.
  Indirect ELISA: To detect hTNF-alpha by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTNF-alpha.
  Sandwich ELISA: To detect hTNF-alpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with biotinylated Anti-Human TNF-alpha as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTNF-alpha.
  Western Blot: To detect hTNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunofluorescence: Recommended to use 10.0 µg/ml for one hour at room temperature.
  Immunohistochemistry on Formalin-fixed, paraffin-embedded sections: The recommended concentrations are 0.5 µg/ml-1.0 µg/ml with an overnight incubation at 4°C. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Human TNF-alpha
• Specificity: Tumor Necrosis Factor alpha
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Lyophilized
• Reconstitution Method: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography employing immobilized hTNF-alpha matrix Sterile filtered.
• Conjugation: Unconjugated
• Storage: The lyophilized antibody can be stored for one year from despatch at -20°C.
  Reconstituted antibody is stable for two weeks at 2-8°C, frozen aliquots are stable for six months at -20°C.
  Avoid repeated freezing and thawing.
• Gene Name: tumor necrosis factor
• Database Link:
  Entrez Gene 7124 Human
  UniProt ID P01375
• Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin