Macrophage Inflammatory Protein 4 (CCL18) Rabbit Polyclonal Antibody

CAT#: PP1054B2

Macrophage Inflammatory Protein 4 (CCL18) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: To detect hMIP-4 by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hMIP-4
  Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hMIP-4.
• Specificity: Specific for human Macrophage Inflammatory Protein-4 (hMIP-4) (PARC).
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: C-C motif chemokine ligand 18
• Database Link:
  Entrez Gene 6362 Human
  UniProt ID P55774
• Background: This chemotactic factor that attracts lymphocytes but not monocytes or granulocytes may be involved in B cell migration into B cell follicles in lymph nodes. It attracts naive T lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T cells, CD4+ and CD8+ T cells and thus may play a role in both humoral and cell-mediated immunity responses. It is expressed at high levels in lung, lymph nodes, placenta, and bone marrow, not expressed by peripheral blood monocytes, and a monocyte-to-macrophage differentiation is a prerequisite for expression. Specifically induced in macrophages by IL4, IL13, and IL10, it belongs to the intercrine beta (chemokine CC) family.
• Synonyms: C-C motif chemokine 18, AMAC1, DCCK1, MIP-4, PARC, Small-inducible cytokine A18, SCYA18
• Note: Centrifuge vial prior to opening!