IL4 Rabbit Polyclonal Antibody

CAT#: PP1026B1

IL4 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: To detect hIL-4 by direct and sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-4.
  Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hIL-4 (human IL-4)
• Specificity: Specific for Human Interleukin-4.
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized purified Ig fraction
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: interleukin 4
• Database Link:
  Entrez Gene 3565 Human
  UniProt ID P05112
• Background: IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
• Synonyms: IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra
• Note: Centrifuge vial prior to opening!