MCP1 (CCL2) Rabbit Polyclonal Antibody

CAT#: PP020B1

MCP1 (CCL2) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Murine JE (MCP-1) by Direct ELISA (using 100 µl/well) solution) a concentration of ~1.0 µg/ml of this antibody is required. This antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Murine JE(MCP-1).
  Sandwich ELISA: To detect Murine JE (MCP-1) by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Purified Anti-Murine JE (MCP-1)(PP020P1, PP020P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine JE(MCP-1).
  Western Blot: To detect Murine JE(MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine JE(MCP-1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Mouse JE (MCP-1).
• Specificity: This antibody reacts with Murine JE (MCP-1).
• Formulation: PBS, pH 7.2 without preservatives.
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction.
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of
  0.1-1.0 mg/ml.
• Purification: Affinity Chromatography.
• Conjugation: Biotin
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: C-C motif chemokine ligand 2
• Database Link:
  UniProt ID P13500
• Background: This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
• Synonyms: C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11
• Note: Centrifuge vial prior to opening!