Ifng Rabbit Polyclonal Antibody

CAT#: PP004P2

Ifng rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine IFN-gamma (1.00 ng/ml), a concentration of 0.05-0.1 µg/ml of this antibody is required.
  Sandwich ELISA: To detect Mouse IFN-gamma by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with biotinylated anti-Murine IFN-gamma (Cat.-No PP004B1 or PP004B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIFN-gamma.
  Western Blot: To detect Mouse IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Murine IFN-gamma (Cat.-No PA007)
• Specificity: Detects Mouse Interferon gamma (IFNg).
  Other species not tested.
• Formulation: PBS, pH 7.2 without additives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge prior to opening.
  Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing immobilized Murine IFN-gamma matrix
• Conjugation: Unconjugated
• Storage: Can be stored lyophilized at 2-8°C for 1 month or at -20°C long term.
• Stability: Shelf life: one year from despatch.
  After reconstitution store the antibody undiluted at 2-8°C for 2 weeks or (in aliquots) at -20°C for up to 6 months.
  Avoid repeated freezing and thawing!
• Gene Name: interferon gamma
• Database Link:
  Entrez Gene 15978 Mouse
  UniProt ID P01580
• Background: Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
• Synonyms: IFN-gamma, gamma IFN