FLT4 Rabbit Polyclonal Antibody

CAT#: DP3511P

FLT4 rabbit polyclonal antibody, Aff - Purified

Product Images

FACS analysis with primary human dermal lymphatic endothelial cells (HDLEC).

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  Immunofluorescence staining (green) of VEGFR-3/FLT4 in primary human dermal lymphatic endothelial cells (HDLEC) with anti-human VEGFR-3/FLT4 (10μg/ml) [Cat#DP3511 and counter staining of nuclei with Dapi. As secondary antibody goat anti-rabbit ALEXA Flour 488 (Dianova) was used 1:800.

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  IHC with cryo sections of human spleen.

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  Western Analysis of anti-human VEGFR-3/FLT-4 Cat.N-DP3511. Samples were loaded in 7.5% SDS-polyacrylamide gel under reducing conditions.

Specifications

Product Data

• Applications: ELISA, FC, IHC, IP, WB
• Recommended Dilution: ELISA: 0.5-1.5 µg/ml, allows the detection of 0.25-0.5 ng/well rhVEGFR-3/FLT-4.
  Western Blot: 0.5-1 µg/ml, it will detect approximately 5 ng/lane of rh VEGFR-3/FLT-4 under reducing conditions depending on the visualisation method.
  Immunoprecipitation: 1-5 µg/ml lysate or reaction volume.
  FACS: Use 1-5 μg/ml
  Immunofluorescence.
  Immunohistochemistry on Frozen sections.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant human soluble FLT-4 protein (110 kDa).
• Specificity: The antibody will detect human VEGFR-3/FLT-4.
  In Western blots, this antibody shows a moderate cross-reactivity with VEGFR-2/KDR and a weak cross-reactivity with VEGFR-1/Flt-1.
• Formulation: PBS, pH 7.4 containing no preservatives or stabilizers
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1 mg/ml.
• Purification: Protein A Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: fms related tyrosine kinase 4
• Database Link:
  Entrez Gene 2324 Human
  UniProt ID P35916
• Background: The sVEGFR-3/FLT-4 monomers have a mass of approximately 120kDa. The soluble receptor protein consists of all 7 extracellular domains (Met1-Glu774). All three VEGF receptors belong to the class III subfamily of receptor tyrosine kinases (RTKs) characterised by the seven immunoglobulin-like loops in the extracellular domain. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. They play key roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The FLT-4 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 23 aa residue signal peptide. Mature VEGFR-3/FLT-4 is composed of a 751 aa residue extracellular domain, a 22 aa transmembrane domain and a 482aa residue cytoplasmic domain. Both VEGF family members VEGF-C and VEGF-D have been shown to bind and activate VEGFR-3/FLT-4. The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial
• Synonyms: VEGFR3, FLT4, VEGF Receptor 3