CD34 Mouse Monoclonal Antibody [Clone ID: 34C02]

CAT#: DM3515P

CD34 mouse monoclonal antibody, clone 34C02, Purified

Specifications

Product Data

• Clone Name: 34C02
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry.
  Immunohistology on Frozen Sections.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human CD34 protein.
• Specificity: This antibody reacts with CD34 - expressed on lymphoid and myeloid hematopoietic progenitor cells.
  Also reacts with vascular endothelial cells in normal tissues and in benign and malignant proliferations.
• Formulation: 10 mM PBS, pH 7.4 without preservatives or stabilizers
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in sterile Water to a concentration of 1.0 mg/ml.
• Purification: Protein G Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD34 molecule
• Database Link:
  Entrez Gene 947 Human
  UniProt ID P28906
• Background: The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
  CD34, a single chain transmembrane glycoprotein, is selectively expressed on human lymphoid and myeloid hematopoietic progenitor cells.
• Synonyms: Hematopoietic progenitor cell marker