VEGF Receptor 1 (FLT1) Mouse Monoclonal Antibody [Clone ID: #EIC]

CAT#: DM3505

VEGF Receptor 1 (FLT1) mouse monoclonal antibody, clone #EIC (formerly FLTEIC), Purified


USD 550.00

2 Weeks*

Size
    • 100 ug

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Specifications

Product Data
Clone Name #EIC
Applications ELISA, FC, IF, WB
Recommended Dilution ELISA: 1-10 µg/ml. 
Western Blot: 2-5 µg/ml.
FACS Analysis and Cell Sorting: The antibody has been used for the detection of Flt-1 positive cells (e.g. PAE/Flt-1 cells).
Immunocytochemistry: 5-30 µg/ml.
Immunoprecipitation: 1-5 µg/ml.
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Recombinant Human soluble extracellular Flt-1 D1-D5 (Cat.-No DA3539).
Specificity The monoclonal antibody will detect Human native and denaturated Human VEGFR-1/ Flt-1 in ELISA experiments, Immunocytochemistry and Cell Sorting.

This clone was formerly named FLTEIC.
Formulation PBS, pH 7.4 containing no preservative
State: Purified
State: Lyophilized purified IgG fraction
Reconstitution Method Restore in distilled sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography on Protein G
Conjugation Unconjugated
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
Synonyms VEGFR1, FLT1, FLT, FRT, VEGF Receptor 1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.