VEGF Receptor 1 (FLT1) Mouse Monoclonal Antibody [Clone ID: #EIC]

CAT#: DM3505

VEGF Receptor 1 (FLT1) mouse monoclonal antibody, clone #EIC (formerly FLTEIC), Purified

Product Images

Western analysis of recombinant human and mouse soluble VEGF receptors using a monoclonal antibody directed against human recombinant sFlt-1 (D5). There is no cross reactivity with mouse sFlt-1 (D7)-Fc as well as with human and mouse sKDR and human sFLT-4 visible.

• 

  Standard ELISA assay with coating of 2.5 ug/ml Mouse sFlt-1-Fc using anti Human Flt-1 antibodies

Specifications

Product Data

• Clone Name: #EIC
• Applications: ELISA, FC, IF, WB
• Recommended Dilution: ELISA: 1-10 µg/ml.
  Western Blot: 2-5 µg/ml.
  FACS Analysis and Cell Sorting: The antibody has been used for the detection of Flt-1 positive cells (e.g. PAE/Flt-1 cells).
  Immunocytochemistry: 5-30 µg/ml.
  Immunoprecipitation: 1-5 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant Human soluble extracellular Flt-1 D1-D5 (Cat.-No DA3539).
• Specificity: The monoclonal antibody will detect Human native and denaturated Human VEGFR-1/ Flt-1 in ELISA experiments, Immunocytochemistry and Cell Sorting.
  
  This clone was formerly named FLTEIC.
• Formulation: PBS, pH 7.4 containing no preservative
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in distilled sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: fms related tyrosine kinase 1
• Database Link:
  Entrez Gene 2321 Human
  UniProt ID P17948
• Background: Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
• Synonyms: VEGFR1, FLT1, FLT, FRT, VEGF Receptor 1