Cytokeratin 5 (KRT5) (+ KRT8) Mouse Monoclonal Antibody [Clone ID: C-50]

CAT#: DM182P

Cytokeratin 5 (KRT5) (+ KRT8) mouse monoclonal antibody, clone C-50, Purified

Product Images

Western Blotting analysis of whole cell lysate of MCF-7 human breast adenocarcinoma cell line. Lane 1: MW standard; Lane 2: immunostaining with anti-cytokeratin 5+18; Lane 3: immunostaining with Isotype mouse IgG1 control

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  Immunohistochemistry staining of human prostate (paraffin section) using anti-cytokeratin 5+18.

Specifications

Product Data

• Clone Name: C-50
• Applications: IF, IHC, IP, WB
• Recommended Dilution: Western blot.
  Immunoprecipitation.
  Immunocytochemistry.
  Immunohistochemistry on Paraffin sections: 10 μg/ml. Positive control: Human prostate.
• Reactivities: Human, Porcine, Mouse, Rat, Hamster, Bovine, Sheep, Mammalian
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cytoskeleton preparation from HeLa cells
• Specificity: The antibody C-50 reacts with Cytokeratin peptides 5 and 8 (58 and 45 kDa).
  Positive Species: Human, Porcine, Mouse, Rat, Hamster, Bovine, Sheep, Canine.
  Negative Species: Chicken, Xenopus. Other not tested
• Formulation: PBS, pH~7.4 with 15 mM Sodium Azide
  State: Purified
  State: Liquid purified IgG fraction (> 95% by SDS-PAGE)
• Concentration: lot specific
• Purification: Protein-A Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: keratin 5
• Database Link:
  Entrez Gene 110308 MouseEntrez Gene 369017 RatEntrez Gene 3852 Human
  UniProt ID P13647
• Background: Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
• Synonyms: KRT5, Cytokeratin-5, Keratin-5, Keratin 5, CK5, K5