Prostate Specific Antigen (KLK3) Mouse Monoclonal Antibody [Clone ID: LT-3D2]

CAT#: DM1225

Prostate Specific Antigen (KLK3) mouse monoclonal antibody, clone LT-3D2, Purified

Product Images

Figure 2. Crified LT-3D2 monoclonal antibody. Lane 1: Molecular Weight marker, Lane 2: purified LT-3D2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.

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  Figure.1: FACS analysis of BOSC23 cells using LT-3D2 antibody. BOSC23 cells were transiently transfected with anexpression vector encoding either PSA (red curve) or anirrelevant protein (Control transfectant: black curve). Binding of LT-3D2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with PSA trans-fected cells.

Specifications

Product Data

• Clone Name: LT-3D2
• Applications: ELISA, FC, WB
• Recommended Dilution: Flow cytometry: 1.2 µg/10⁶ cells.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Genetic immunisation with cDNA encoding Human PSA
• Specificity: Recognizes Human PSA (glandular Kallikrein 3).
  Other species not tested.
• Formulation: Phosphate buffered saline, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: kallikrein related peptidase 3
• Database Link:
  Entrez Gene 354 Human
  UniProt ID P07288
• Background: Prostate specific antigen (PSA) belongs to the kallikrein gene family. It is a single-chain glycoprotein with a molecular mass of about 33 kD which is produced by prostate epithelial cells. It is secreted in the seminal plasma and is responsible for liquefaction of the seminal coagulum (1,2). PSA is present in small quantities in the serum of healthy males, and is often elevated in the presence of prostate cancer and in other prostate disorders. It is one of the most useful tumour markers because of its tissue specificity. It is thus widely used for screening, diagnosis, and monitoring patients with prostate cancer (3).
• Synonyms: Prostate-specific antigen, Semenogelase, Gamma-seminoprotein, P-30 antigen
• Note: SDS-PAGE analysis of LT-3D2: The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Figure.2).

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Pathways in cancer, Prostate cancer