Chromogranin A (CHGA) Mouse Monoclonal Antibody [Clone ID: NJ-5C9]

CAT#: DM1220

Chromogranin A (CHGA) mouse monoclonal antibody, clone NJ-5C9, Purified

Product Images

Figure 2. SDS-PAGE analysis of purified NJ-5C9 antibody. Lane 1: Molecular weight marker, Lane 2: 2ug of purified NJ-5C9 antibody. Proteins were separated by CGE (Capillary Gel Electrophoresis, Agilent 2100 Bioanalyzer). Internal Control bands (240 kDa / 7 kDa / 4.5 kDa).

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  Figure.1: FACS analysis of BOSC23 cells using NJ-5C9. BOSC23 cells were transiently transfected with anexpression vector encoding either CGA (red curve) or anirrelevant protein (Control transfectant: black curve). Binding of NJ-5C9 was detected with a PE-conjugated secondaryantibody. A positive signal was obtained only with CGA trans-fected cells.

Specifications

Product Data

• Clone Name: NJ-5C9
• Applications: ELISA, FC, WB
• Recommended Dilution: Flow cytometry: 1.2 µg/10e6 cells.
  ELISA: 1/200-1/400.
  Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Genetic immunisation with cDNA encoding CGA
• Specificity: Recognizes Chromogranin A (CGA).
• Formulation: Phosphate buffered saline, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: chromogranin A
• Database Link:
  Entrez Gene 12652 MouseEntrez Gene 1113 Human
  UniProt ID P10645
• Background: Chromogranin A (CgA) is a 49 kDa protein which is a member of the granin/secretogranin family of acidic glycoproteins. It is specifically expressed in all endocrine and neuroendocrine cells and plays multiple roles in the process of regulated secretion of peptide hormones and neurotransmitters (1). Owing to its over-expression in several tumors of neuroendocrine origin, CgA is of diagnostic value in classical endocrine, in hormone-negative and in neuroendocrine tumors (2).
• Synonyms: Chromogranin-A, CgA, CHGA, SP-I

Reference Data

• Protein Families: Druggable Genome, Secreted Protein