TGF beta 1 (TGFB1) Mouse Monoclonal Antibody [Clone ID: 2C6]

CAT#: DM1045

TGF beta 1 (TGFB1) mouse monoclonal antibody, clone 2C6, Azide Free

Specifications

Product Data

• Clone Name: 2C6
• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: ELISA.
  Western Blot: MAb 2C6 at a concentration of 50-500 ng/mL will allow visualization of 100-500 ng/lane of TGF-β.
  Neutralization: This MAb neutralizes TGF-β activity in vitro in inhibition assay of CCL/64 cell growth and also neutralize the growth promoting action of TGF-β in the NRK-49F colony forming assays. The effect of micro-injection of this MAb into one blastomere of two cell stage Xenopus embryos indicated that this MAb was able to neutralize effectively the bioactivity of TGF-β in vivo.
  Immunohistochemistry: May be used in immunohistochemical techniques to locate TGF-β within tissues.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: TGF-β from human platelets
• Specificity: Western blotting demonstrated that this MAb reacts to either dimeric (25 kDa) or monomeric (12.5 kDa) molecules of TGF-β under non-reducing and reducing condition respectively.
  This MAb recognizes both human platelet-derived and recombinant TGF-β in enzyme-linked immunosorbent assay (ELISA).
• Formulation: 0.01M PBS, pH 7.0
  State: Azide Free
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with double distillated water to adjust the final concentration to 1.0 mg/ml.
• Purification: Protein G affinity purified
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: transforming growth factor beta 1
• Database Link:
  Entrez Gene 7040 Human
  UniProt ID P01137
• Background: TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function.
  The C-terminal 112 amino acids constitute the mature protein.
• Synonyms: TGFB, Transforming growth factor beta-1, TGF-beta-1