IL8 (CXCL8) Mouse Monoclonal Antibody [Clone ID: I8-60]

CAT#: DM1023

IL8 (CXCL8) mouse monoclonal antibody, clone I8-60, Azide Free

Product Images

Western blot analysis of Human IL-8 using anti-human IL8 Antibody

Specifications

Product Data

• Clone Name: I8-60
• Applications: ELISA, Neutralize, WB
• Recommended Dilution: ELISA: Can be used as a Tracer antibody in Sandwich ELISA for Detection of Human IL-8 (matched pair with clone I8-S2, Cat.-No DM1022 as Capture antibody)
  Suggested Capture Coating Dose: 0.3 µg/well, substrate TMB.
  Neutralizing: In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5 µg/ml of MAb and 10 ng/ml of Human IL-8 in assay. This MAb inhibited the chemotactic effect of Human IL-8, had no inhibitory effect to MIP 1-beta and RANTES in neutrophil chemotaxis assay. It indicates the MAb is special neutralizing the IL-8 activity. The MAb has been shown the neutralizing activity on calcium ion changes in human granulocytes using FACS.
  Western Blot: Concentration of 0.02-0.1 µg/ml MAb will allow visualization of 100 ng/lane of Human IL-8.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant human IL-8.
• Specificity: This monoclonal antibody recognizes both recombinant and native Human Interleukin-8.
  This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
• Formulation: 0.01M PBS, pH 7.0
  State: Azide Free
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
• Concentration: 1.0 mg/ml (after reconstitution)
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: C-X-C motif chemokine ligand 8
• Database Link:
  Entrez Gene 3576 Human
  UniProt ID P10145
• Background: Interleukin 8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the C-X-C chemokine family. IL-8 has 4 cysteine residues, as do other members of the chemokine family, and the first two cysteine residues are separated by glutamine. IL-8 consists of 72 amino acids with a molecular weight of 8,000 daltons. IL-8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils. IL-8 activates neutrophils to release lysosomal enzymes including myeloperoxidase, -mannosidase and -glucuronidase. IL-1 induces the production of IL-8 from fibroblasts, keratinocytes, endothelial cells, hepatoma cells, astrocytoma cells, glioblastoma cells, lung epithelial cells, synovial membrane cells, melanocytes, melanoma cells and gastric cancer cells. Lipopolysaccharides will stimulate IL-8 production in monocytes/macrophages, and expressed on endothelial cells in response to inflammation.
• Synonyms: CXCL8, Protein 3-10C, Emoctakin, GCP1, MDNCF, MONAP, NAP1