CD49b (ITGA2) Human Monoclonal Antibody [Clone ID: F2H11]

CAT#: DDX9021A488-100

CD49b (ITGA2) human monoclonal antibody, clone F2H11

Product Images

IF stainingof HPA5b + platelets with F2H11

• 

  ACS staining of HPA5 + platelets with F2H11

Specifications

Product Data

• Clone Name: F2H11
• Applications: FC, IF
• Recommended Dilution: Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F) .
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-25μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
  
• Reactivities: Human
• Host: Human
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen:
  CD40 activated and EBV immortalized human B lymphocytes
• Specificity: HPA5b (does not bind to HPA5a differing by a single amino acid).
• Formulation:
  PBS, 50% glycerol
  
  Label: Alexa Fluor 488
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 488
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: integrin subunit alpha 2
• Database Link:
  Entrez Gene 3673 Human
  UniProt ID P17301
• Background: Neonatal AlloImmune Thrombocytopenia (NAIT) is the commonest cause of severe isolated thrombocytopenia in the foetus and newborn. In Cauc asians, 97% of NAIT are caused by HPA-1a or HPA- 5b alloantibodies. By combining CD40 activation and Epstein-Barr virus transformation of B cells isola ted from a woman with high titre of anti -HPA-5b alloan tibodies. F2H11 human monoclonal antibody is highly specific (does not bind to HPA5a) , and allows a r apid and accurate phenotyping of pregnant women as well as selection of blood donors. (Mérieux Y, Rigal D, Pin JJ, XVII th Regional Congress, International Society of Blood Transfusion, Madrid 2007 ; Guillot -Chene P, Lebecque S, Rigal D, Annales Pharmaceutiques Françaises, 06/2009, 67 (3) 182-6).
• Synonyms: human platelet antigen 5b