MYD88 Mouse Monoclonal Antibody [Clone ID: 603E10.06]

CAT#: DDX1401P-100

MYD88 mouse monoclonal antibody, clone 603E10.06

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Specifications

Product Data

• Clone Name: 603E10.06
• Applications: FC, IF
• Recommended Dilution: DDX1401P-50 / DDX1401P-100 Purified: FACS intracellular, ImmunoFluorescence.
  DDX1401A488-50 / DDX1401A488-100 Alexa- fluor®A488: FACS intracellular, ImmunoFluorescence.
  DDX1401A546-50 / DDX1401A546-100 Alexa- fluor®546: FACS intracellular, ImmunoFluorescence.
  DDX1401A647-50 / DDX1401A647-100 Alexa- fluor®647: FACS intracellular, ImmunoFluorescence.
  
  Applications tested: Intracyto flow cytometry, IF, IHC paraffin.
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before using.
  
• Reactivities: Canine, Human, Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: huMyd88 transfected 293T cells
• Specificity: Human Myd88.
  Both 603E10.05 and 603E10.06 monoclonal antibodies were validated both in Human and in Dog. 603E10.05 was validated in Swine.
  Species cross-reactivity: Negative for Murine Myd88.
• Formulation: Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
  State: Purified
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: myeloid differentiation primary response 88
• Database Link:
  Entrez Gene 4615 Human
  UniProt ID Q99836
• Background: MyD88 (myeloid differentiation primary response gene 88) is a universal adapter protein of 296 aa used by all TLRs (except TLR3) and by IL1-R to activate the transcription factor NF-kB. Myd88 binds IRAK1, IRAK2 and TRAF6, leading to NF-kB activation, cytokine secretion and inflammatory response. Myd88 increases IL8 transcription, and is involved in IL18 signaling pathway. A monoclonal antibody was raised against huMyd88-transfected 293T cells. This antibody specifically recognizes endogenously expressed Myd88 and does not cross-react with the mouse Myd88. Both 603E10.05 and 603E10.06 monoclonal antibodies were validated both in human and in dog. 603E10.05 was validated in swine.
• Synonyms: Myeloid differentiation primary response protein MyD88