IL17 (IL17A) Mouse Monoclonal Antibody [Clone ID: 412H6.03]

CAT#: DDX0339P-100

IL17 (IL17A) mouse monoclonal antibody, clone 412H6.03

Specifications

Product Data

• Clone Name: 412H6.03
• Applications: FC
• Recommended Dilution: DDX0339P-50 DDX0339P-100 Purified: FACS intracellular.
  Usage recommendation:
  *This monoclonal antibody may be used between 1-10 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: rhCytotoxic T Lymphocyte associated-Antigen 8 transfected COS-7 cells.
• Specificity: Human IL-17A.
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
  State: Purified
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: interleukin 17A
• Database Link:
  Entrez Gene 3605 Human
  UniProt ID Q16552
• Background: IL-17 (cytotoxic T lymphocyte associated antigen 8) is a CD4+ T cell-derived cytokine that stimulates stromal cells and macrophages to secrete proinflammatory cytokines. To address a possible mechanism by which IL-17 may promote alloreactivity, we examined the influence of IL-17 on the differentiation and function of bone marrow-derived cells propagated in GM-CSF with or without IL-4 to promote dendritic cell (DC) growth. A minor proportion of CD11c+ DC expressed the IL-17R. IL-17 promoted the maturation of DC progenitors, as evidenced by increased cell surface expression of CD11c, costimulatory molecules (CD40, CD80, CD86), and MHC class II Ag, and allostimulatory capacity. IL-17 had a lesser effect on the phenotype and function of more fully differentiated myeloid DC. These findings suggest a role for IL-17 in allogeneic T cell proliferation that may be mediated in part via a maturation-inducing effect on DC. IL-17 appears to be a novel target for therapeutic intervention in allograft rejection. hIL17 stimulate epithelial, endothelial, and fibroblastic cells to secrete cytokines such as IL-6, IL-8, and G-CSF and PGE2. (Fossiez F et al, 1996 ; J. Exp. Med., 183:2593-2603 ; Fossiez F. et al, 1998 ; Int. Rev. Immunol., 16:541-551).
• Synonyms: IL-17A, IL17, IL-17, CTLA8, CTLA-8