Interleukin-4 / IL4 Rat Monoclonal Antibody [Clone ID: 11B4]

CAT#: DDX0311P-100

Interleukin-4 / IL4 rat monoclonal antibody, clone 11B4

Specifications

Product Data

• Clone Name: 11B4
• Applications: ELISA, IP, Neutralize
• Recommended Dilution: ELISA, IP, Neutralization of IL4 bioactivity.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human IL4-transfected Cos7 cells.
• Specificity: Human interleukin-4.
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
  State: Purified
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Database Link:
  NP_000580.1
  Entrez Gene 3565 Human
• Background: Interleukin-4 (IL-4) is a monomeric 20 kDa predominantly produced by activated T lymphocytes of the Th2 phenotype, by mast cells and by some antigen-presenting cells. Together with IL-2, IL-4 is an autocrine growth factor for activated T cells and for cytotoxic T cell precursors. IL-4 is a major factor involved in the activation of resting B lymphocytes. Together with IL-13, IL-4 induces isotype switch towards IgE and IgG1 isotypes. 11B4, was produced from a rat immunized with purified human IL4-transfected-COS7 cells. It recognized both E. coli-expressed and mammalian cell-expressed (COS7 and L cell), rhuIL-4 in solution (immunoprecipitation), as well as on solid phase (indirect ELISA and dot-blotting). The 11B4 antibody inhibited IL-4 bioactivity at an IC50 which was 25-50-fold in molar excess of factor. The 11B4 antibody was used to develop an immunoenzymatic assay capable of detecting concentrations less than 100 pg/ml. (Chrétien I et al, 1989, J. Immunol. Methods, 1178 (1) : 67-81; Ramanathan L.et al, 1993, Biochemistry, 32 (14) :3549-56).
• Synonyms: IL-4, BSF1, Lymphocyte stimulatory factor 1, Binetrakin, Pitrakinra

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Allograft rejection, Asthma, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, T cell receptor signaling pathway