Cd8a Rat Monoclonal Antibody [Clone ID: CT-CD8a]

CAT#: CL008APC

Cd8a rat monoclonal antibody, clone CT-CD8a, APC

Product Images

Cell Source: CD3+ Spleen cells Percentage of cells stained above control: 13.1%

Specifications

Product Data

• Clone Name: CT-CD8a
• Applications: FC
• Recommended Dilution: Flow Cytometry Analysis (see Protocols).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This anti-mouse CD8a antigen monoclonal antibody recognizes the mouse CD8α chain. The α chain of CD8 associates with the CD8β chain to form a CD8α/βheterodimer that is expressed by the majority of thymocytes and by the MHC class I restricted subset of mature T cells1. Mouse CD8α can also form a CD8α/α chain homodimer on subsets of CD8 positive T cells. For this reason, antibodies specific for CD8a rather than CD8b are recommended for a rigorous delineation of CD8 positive cells.
• Formulation: 0.5 ml PBS and 0.1% sodium azide (NaN3). A highly purified grade of BSA has been added as a stabilizing protein to bring the final protein concentration to 4-5 mg/ml after conjugation.
  Label: APC
  State: Liquid purified IgG
• Concentration: lot specific
• Conjugation: APC
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be proteced from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD8 antigen, alpha chain
• Database Link:
  Entrez Gene 12525 Mouse
  UniProt ID P01731
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL
• Note: Protocol: FLOW CYTOMETRY ANALYSIS:
  
  Method:
  1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
  2. Wash 2 times.
  3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
  4. To each tube, add ~0.5 µg* of this Ab.
  5. Vortex the tubes to ensure thorough mixing of antibody and cells.
  6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive.)
  7. Wash 2 times at 4°C.
  8. Resuspend the cell pellet in 50 µl ice cold media B.
  9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.
  
  Media:
  A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 µl of 2M sodium azide in 100 mls).
  B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 µl of 2M sodium azide in 100 mls).
  
  Results:
  Mouse Strain: BALB/c
  Cell Concentration: 1 x 10e6 cells per test
  Antibody Concentration Used: 0.5 μg/10e6 cells
  Isotypic Control: APC Rat IgG2a