BRCC36 (BRCC3) Mouse Monoclonal Antibody [Clone ID: OTI6G7]

CAT#: CF811409

Carrier-free (BSA/glycerol-free) BRCC3 mouse monoclonal antibody,clone OTI6G7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BRCC3 (Cat# RC200727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRCC3 (Cat# TA811409)(1:500). Positive lysates LY411291 (100ug) and LC411291 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6G7
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 41-316 of human BRCC3 (NP_077308) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BRCA1/BRCA2-containing complex subunit 3
• Database Link:
  NP_077308
  Entrez Gene 210766 MouseEntrez Gene 316794 RatEntrez Gene 79184 Human
  UniProt ID P46736
• Background: This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011]
• Synonyms: BRCC36; C6.1A; CXorf53

Reference Data

• Protein Families: Druggable Genome, Protease