ADCK3 (COQ8A) Mouse Monoclonal Antibody [Clone ID: OTI2A7]

CAT#: CF810996

Carrier-free (BSA/glycerol-free) ADCK3 mouse monoclonal antibody,clone OTI2A7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ADCK3 (Cat# RC201457, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADCK3(Cat# TA810996). Positive lysates LY402769 (100ug) and LC402769 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2A7
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 57-328 of human ADCK3 (NP_064632) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71.8 kDa
• Gene Name: coenzyme Q8A
• Database Link:
  NP_064632
  Entrez Gene 56997 Human
  UniProt ID Q8NI60
• Background: This gene encodes a mitochondrial protein similar to yeast ABC1, which functions in an electron-transferring membrane protein complex in the respiratory chain. It is not related to the family of ABC transporter proteins. Expression of this gene is induced by the tumor suppressor p53 and in response to DNA damage, and inhibiting its expression partially suppresses p53-induced apoptosis. Alternatively spliced transcript variants have been found; however, their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: ARCA2; CABC1; COQ8; COQ10D4; SCAR9

Reference Data

• Protein Families: Protein Kinase