PRRT2 Mouse Monoclonal Antibody [Clone ID: OTI9H8]

CAT#: CF809867

Carrier-free (BSA/glycerol-free) PRRT2 mouse monoclonal antibody, clone OTI9H8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PRRT2 (Cat# RC202304, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRRT2 (Cat# TA809867)(1:500).

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  Western blot analysis of extracts (35ug) from mouse brain tissue lysate by using anti-PRRT2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue within the normal limits using anti-PRRT2 mouse monoclonal antibody.This figure shows negative staining. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809867) (1:150) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-PRRT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809867) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using anti-PRRT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809867) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human placenta tissue within the normal limits using anti-PRRT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809867) (1:2000)

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Specifications

Product Data

• Clone Name: OTI9H8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 152-268 of human PRRT2(NP_660282) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: proline rich transmembrane protein 2
• Database Link:
  NP_660282
  Entrez Gene 112476 Human
  UniProt ID Q7Z6L0
• Background: This gene encodes a transmembrane protein containing a proline-rich domain in its N-terminal half. Studies in mice suggest that it is predominantly expressed in brain and spinal cord in embryonic and postnatal stages. Mutations in this gene are associated with episodic kinesigenic dyskinesia-1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
• Synonyms: BFIC2; BFIS2; DSPB3; DYT10; EKD1; FICCA; ICCA; IFITMD1; PKC

Reference Data

• Protein Families: Transmembrane