POU4F3 Mouse Monoclonal Antibody [Clone ID: OTI6F4]

CAT#: CF808756

Carrier-free (BSA/glycerol-free) POU4F3 mouse monoclonal antibody, clone OTI6F4 (formerly 6F4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU4F3 (RC211206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU4F3 (1:2000). Positive lysates LY400949 (100ug) and LC400949 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6F4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 179-338 of human POU4F3(NP_002691) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.9 kDa
• Gene Name: POU class 4 homeobox 3
• Database Link:
  NP_002691
  Entrez Gene 18998 MouseEntrez Gene 364855 RatEntrez Gene 5459 Human
  UniProt ID Q15319
• Background: This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. [provided by RefSeq, Mar 2009]
• Synonyms: BRN3C; DFNA15

Reference Data

• Protein Families: Transcription Factors