MCEE Mouse Monoclonal Antibody [Clone ID: OTI11G7]

CAT#: CF808608

Carrier-free (BSA/glycerol-free) MCEE mouse monoclonal antibody, clone OTI11G7 (formerly 11G7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCEE (RC205018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCEE (1:2000). Positive lysates LY403176 (100ug) and LC403176 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808608) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808608) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808608) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808608) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808608) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MCEE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808608) (1:150)

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Specifications

Product Data

• Clone Name: OTI11G7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MCEE (NP_115990) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: methylmalonyl-CoA epimerase
• Database Link:
  NP_115990
  Entrez Gene 84693 Human
  UniProt ID Q96PE7
• Background: The product of this gene catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids. odd chain-length fatty acids, and other metabolites. Mutations in this gene result in methylmalonyl-CoA epimerase deficiency, which is presented as mild to moderate methylmalonic aciduria. [provided by RefSeq, Jul 2008]
• Synonyms: GLOD2

Reference Data

• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation