EGFL7 Mouse Monoclonal Antibody [Clone ID: OTI2B5]

CAT#: CF807830

Carrier-free (BSA/glycerol-free) EGFL7 mouse monoclonal antibody, clone OTI2B5 (formerly 2B5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGFL7 (RC218849, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGFL7 (1:2000). Positive lysates LY402519 (100ug) and LC402519 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2B5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 24-273 of human EGFL7(NP_057299) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.4 kDa
• Gene Name: EGF like domain multiple 7
• Database Link:
  NP_057299
  Entrez Gene 51162 Human
  UniProt ID Q9UHF1
• Background: This gene encodes a secreted endothelial cell protein that contains two epidermal growth factor-like domains. The encoded protein may play a role in regulating vasculogenesis. This protein may be involved in the growth and proliferation of tumor cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]
• Synonyms: NEU1; VE-STATIN; ZNEU1

Reference Data

• Protein Families: Secreted Protein