Fascin (FSCN1) Mouse Monoclonal Antibody [Clone ID: OTI5G1]

CAT#: CF807305

Carrier-free (BSA/glycerol-free) FSCN1 mouse monoclonal antibody, clone OTI5G1 (formerly 5G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FSCN1 (RC203031, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FSCN1 (1:2000). Positive lysates LY401076 (100ug) and LC401076 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807305) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807305) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FSCN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807305) (1:150)

Specifications

Product Data

• Clone Name: OTI5G1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FSCN1 (NP_003079) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.3 kDa
• Gene Name: fascin actin-bundling protein 1
• Database Link:
  NP_003079
  Entrez Gene 14086 MouseEntrez Gene 683788 RatEntrez Gene 6624 Human
  UniProt ID Q16658
• Background: This gene encodes a member of the fascin family of actin-binding proteins. Fascin proteins organize F-actin into parallel bundles, and are required for the formation of actin-based cellular protrusions. The encoded protein plays a critical role in cell migration, motility, adhesion and cellular interactions. Expression of this gene is known to be regulated by several microRNAs, and overexpression of this gene may play a role in the metastasis of multiple types of cancer by increasing cell motility. Expression of this gene is also a marker for Reed-Sternberg cells in Hodgkin's lymphoma. A pseudogene of this gene is located on the long arm of chromosome 15. [provided by RefSeq, Sep 2011]
• Synonyms: FAN1; HSN; p55; SNL