RET Mouse Monoclonal Antibody [Clone ID: OTI20B8]

CAT#: CF805940

Carrier-free (BSA/glycerol-free) RET mouse monoclonal antibody, clone OTI20B8 (formerly 20B8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RET (RC214268, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RET (1:500).

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  Immunohistochemical staining of paraffin-embedded RET positive Human lung carcinoma tissue using anti-RET mouse monoclonal antibody. (TA805940; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded RET positive Human lung carcinoma tissue using anti-RET mouse monoclonal antibody. (TA805940; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Specifications

Product Data

• Clone Name: OTI20B8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 713-1017 of human RET(NP_066124) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 124.1 kDa
• Gene Name: ret proto-oncogene
• Database Link:
  NP_066124
  Entrez Gene 24716 RatEntrez Gene 5979 Human
  UniProt ID P07949
• Synonyms: CDHF12; CDHR16; HSCR1; MEN2A; MEN2B; MTC1; PTC; RET-ELE1; RET51

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Endocytosis, Pathways in cancer, Thyroid cancer