Estrogen Related Receptor beta (ESRRB) Mouse Monoclonal Antibody [Clone ID: OTI12H2]

CAT#: CF805749

Carrier-free (BSA/glycerol-free) ESRRB mouse monoclonal antibody, clone OTI12H2 (formerly 12H2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ESRRB (Cat# RC215995, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ESRRB(Cat# TA805749). Positive lysates LY432280 (100ug) and LC432280 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI12H2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-252 of human ESRRB (NP_004443) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56 kDa
• Gene Name: estrogen related receptor beta
• Database Link:
  NP_004443
  Entrez Gene 26380 MouseEntrez Gene 299210 RatEntrez Gene 2103 Human
  UniProt ID O95718
• Background: This gene encodes a protein with similarity to the estrogen receptor. Its function is unknown; however, a similar protein in mouse plays an essential role in placental development. [provided by RefSeq, Jul 2008]
• Synonyms: DFNB35; ERR2; ERRb; ESRL2; NR3B2

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors