TWIST2 Mouse Monoclonal Antibody [Clone ID: OTI9F11]

CAT#: CF805301

Carrier-free (BSA/glycerol-free) TWIST2 mouse monoclonal antibody, clone OTI9F11 (formerly 9F11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TWIST2 (RC205006, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TWIST2. Positive lysates LY403298 (100ug) and LC403298 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI9F11
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TWIST2 (NP_476527) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.9 kDa
• Gene Name: twist family bHLH transcription factor 2
• Database Link:
  NP_476527
  Entrez Gene 13345 MouseEntrez Gene 59327 RatEntrez Gene 117581 Human
  UniProt ID Q8WVJ9
• Background: Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2012]
• Synonyms: AMS; BBRSAY; bHLHa39; DERMO1; FFDD3; SETLSS

Reference Data

• Protein Families: Druggable Genome, Transcription Factors