SGK196 (POMK) Mouse Monoclonal Antibody [Clone ID: OTI2B10]

CAT#: CF804622

Carrier-free (glycerol/BSA-free) SGK196 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SGK196 (RC222797, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SGK196. Positive lysates LY410261 (100ug) and LC410261 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SGK196 Mouse monoclonal antibody. (TA804622; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Specifications

Product Data

• Clone Name: OTI2B10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 141-350 of human SGK196 (NP_115613) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.9 kDa
• Gene Name: protein O-mannose kinase
• Database Link:
  NP_115613
  Entrez Gene 84197 Human
  UniProt ID Q9H5K3
• Background: This gene encodes a protein that may be involved in the presentation of the laminin-binding O-linked carbohydrate chain of alpha-dystroglycan (a-DG), which forms transmembrane linkages between the extracellular matrix and the exoskeleton. Some pathogens use this O-linked carbohydrate unit for host entry. Loss of function compound heterozygous mutations in this gene were found in a human patient affected by the Walker-Warburg syndrome (WWS) phenotype. Mice lacking this gene contain misplaced neurons (heterotopia) in some regions of the brain, possibly from defects in neuronal migration. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
• Synonyms: MDDGA12; MDDGC12; SGK196

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane