ADAMTS1 Mouse Monoclonal Antibody [Clone ID: OTI4H2]

CAT#: CF804503

Carrier-free (BSA/glycerol-free) ADAMTS1 mouse monoclonal antibody, clone OTI4H2 (formerly 4H2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADAMTS1 (RC205984, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADAMTS1. Positive lysates LY416271 (100ug) and LC416271 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ADAMTS1 mouse monoclonal antibody. (TA804503) Dilution: 1:150

Specifications

Product Data

• Clone Name: OTI4H2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 412-644 of human ADAMTS1 (NP_008919) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 100.3 kDa
• Gene Name: ADAM metallopeptidase with thrombospondin type 1 motif 1
• Database Link:
  NP_008919
  Entrez Gene 9510 Human
  UniProt ID Q9UHI8
• Background: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. [provided by RefSeq, Jul 2008]
• Synonyms: C3-C5; METH1

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein