C10orf63 (ENKUR) Mouse Monoclonal Antibody [Clone ID: OTI3H10]

CAT#: CF804182

Carrier-free (BSA/glycerol-free) ENKUR mouse monoclonal antibody, clone OTI3H10 (formerly 3H10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENKUR (RC205483, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ENKUR. Positive lysates LY408146 (100ug) and LC408146 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3H10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ENKUR (NP_659447) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.3 kDa
• Gene Name: enkurin, TRPC channel interacting protein
• Database Link:
  NP_659447
  Entrez Gene 219670 Human
  UniProt ID Q8TC29
• Background: This gene encodes a protein that interacts with calmodulin and several transient receptor potential canonical cation channel proteins. The encoded protein may function as an adaptor to localize signal transduction machinery to calcium channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
• Synonyms: C10orf63; CFAP106