APC Mouse Monoclonal Antibody [Clone ID: OTI1B12]

CAT#: CF803682

Carrier-free (BSA/glycerol-free) APC mouse monoclonal antibody, clone OTI1B12 (formerly 1B12)

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-APC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803682)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-APC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803682)

Specifications

Product Data

• Clone Name: OTI1B12
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 955-1382 of human APC (NP_001120983) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 311.5 kDa
• Gene Name: APC regulator of WNT signaling pathway
• Database Link:
  NP_001120983
  Entrez Gene 11789 MouseEntrez Gene 24205 RatEntrez Gene 324 Human
  UniProt ID P25054
• Synonyms: BTPS2; DP2; DP2.5; DP3; GS; PPP1R46

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Pathways in cancer, Regulation of actin cytoskeleton, Wnt signaling pathway