Suppressor of Ty 4 homolog 1 (SUPT4H1) Mouse Monoclonal Antibody [Clone ID: OTI5F12]

CAT#: CF803431

Carrier-free (BSA/glycerol-free) SUPT4H1 mouse monoclonal antibody, clone OTI5F12 (formerly 5F12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SUPT4H1 (Cat# RC201211, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUPT4H1(Cat# TA803431). Positive lysates LY418857 (100ug) and LC418857 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5F12
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SUPT4H1 (NP_003159) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13 kDa
• Gene Name: SPT4 homolog, DSIF elongation factor subunit
• Database Link:
  NP_003159
  Entrez Gene 287608 RatEntrez Gene 6827 Human
  UniProt ID P63272
• Background: This gene encodes the small subunit of DRB (5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
• Synonyms: SPT4; SPT4H; Supt4a; SUPT4H

Reference Data

• Protein Families: Transcription Factors