RIP3 (RIPK3) Mouse Monoclonal Antibody [Clone ID: OTI3A3]

CAT#: CF803231

Carrier-free (BSA/glycerol-free) RIPK3 mouse monoclonal antibody, clone OTI3A3 (formerly 3A3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3 (RC209549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPK3.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-RIPK3 monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RIPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803231)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RIPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803231)

Specifications

Product Data

• Clone Name: OTI3A3
• Applications: IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 233-518 of human RIPK3 (NP_006862) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.7 kDa
• Gene Name: receptor interacting serine/threonine kinase 3
• Database Link:
  NP_006862
  Entrez Gene 11035 Human
  UniProt ID Q9Y572
• Background: The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]
• Synonyms: RIP3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cytosolic DNA-sensing pathway