KRAS Mouse Monoclonal Antibody [Clone ID: OTI2C1]

CAT#: CF801672

Carrier-free (BSA/glycerol-free) KRAS mouse monoclonal antibody, clone OTI2C1 (formerly 2C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KRAS (Cat# RC222697, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS(Cat# TA801672). Positive lysates LY409579 (100ug) and LC409579 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRAS monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

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  Immunohistochemical staining of paraffin-embedded human normal colon using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. TA801672 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show nuclear and cytoplasmic staining.

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  Immunohistochemical staining of paraffin-embedded human normal brain using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. TA801672 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show nuclear and cytoplasmic staining.

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  Immunohistochemical staining of paraffin-embedded human normal brain using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. TA801672 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show nuclear and cytoplasmic staining.

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  Immunohistochemical staining of paraffin-embedded human normal kidney using anti-KRAS clone OTI2C1 mouse monoclonal antibody @ 1:100 with D12-18 Polink1 anti-Ms HRP DAB detection kit. TA801672 tissue screen used heat-induced epitope retrieval buffer ACCEL, pH8.7 in pressure cooker. Results show mostly cytoplasmic staining.

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Specifications

Product Data

• Clone Name: OTI2C1
• Applications: IHC, WB
• Recommended Dilution: IHC 1:100, WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KRAS (NP_203524) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.5 kDa
• Gene Name: Homo sapiens KRAS proto-oncogene, GTPase (KRAS), transcript variant a, mRNA.
• Database Link:
  NP_203524
  Entrez Gene 16653 MouseEntrez Gene 24525 RatEntrez Gene 3845 Human
  UniProt ID P01116
• Background: This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
• Synonyms: C-K-RAS; c-Ki-ras2; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway