Collagen XV (COL15A1) Mouse Monoclonal Antibody [Clone ID: OTI1C5]

CAT#: CF507124

Carrier-free (BSA/glycerol-free) COL15A1 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY COL15A1 (Cat# RC215981, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-COL15A1(Cat# TA507124). Positive lysates LY419713 (100ug) and LC419713 (20ug) can be purchased separately from OriGene.

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  Anti-COL15A1 mouse monoclonal antibody (TA507124) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY COL15A1 (RC215981).

Specifications

Product Data

• Clone Name: OTI1C5
• Applications: IF, WB
• Recommended Dilution: WB 1:4000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human COL15A1(NP_001846) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 138.9 kDa
• Gene Name: collagen type XV alpha 1 chain
• Database Link:
  NP_001846
  Entrez Gene 1306 Human
  UniProt ID P39059
• Background: This gene encodes the alpha chain of type XV collagen, a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Type XV collagen has a wide tissue distribution but the strongest expression is localized to basement membrane zones so it may function to adhere basement membranes to underlying connective tissue stroma. Mouse studies have shown that collagen XV deficiency is associated with muscle and microvessel deterioration. [provided by RefSeq, Jul 2008]
• Synonyms: FLJ38566