Placental lactogen (CSH1) Mouse Monoclonal Antibody [Clone ID: OTI2A2]

CAT#: CF506813

Carrier-free (BSA/glycerol-free) CSH1 mouse monoclonal antibody, clone OTI2A2 (formerly 2A2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSH1 (RC223244, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSH1. Positive lysates LY411615 (100ug) and LC411615 (20ug) can be purchased separately from OriGene.

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  Anti-CSH1 mouse monoclonal antibody (TA506813) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CSH1 (RC223244).

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  Immunofluorescent staining of HeLa cells using anti-CSH1 mouse monoclonal antibody (TA506813).

Specifications

Product Data

• Clone Name: OTI2A2
• Applications: IF, WB
• Recommended Dilution: WB 1:1000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CSH1(NP_072166) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: chorionic somatomammotropin hormone 1
• Database Link:
  NP_072166
  Entrez Gene 1442 Human
  UniProt ID P0DML2
• Background: The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites. Expression of the identical mature proteins for chorionic somatomammotropin hormones 1 and 2 is upregulated during development, although the ratio of 1 to 2 increases by term. Mutations in this gene result in placental lactogen deficiency and Silver-Russell syndrome. [provided by RefSeq, Jul 2008]
• Synonyms: CSA; CSH2; CSMT; FLJ75407; PL

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction