RFC4 Mouse Monoclonal Antibody [Clone ID: OTI1A8]

CAT#: CF506183

Carrier-free (BSA/glycerol-free) RFC4 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RFC4 (Cat# RC200426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC4(Cat# TA506183). Positive lysates LY419019 (100ug) and LC419019 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RFC4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506183)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506183)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506183)

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  Anti-RFC4 mouse monoclonal antibody (TA506183) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFC4 (RC200426).

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Specifications

Product Data

• Clone Name: OTI1A8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200~4000, IHC 1:150, IF 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RFC4(NP_002907) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.5 kDa
• Gene Name: replication factor C subunit 4
• Database Link:
  NP_002907
  Entrez Gene 106344 MouseEntrez Gene 288003 RatEntrez Gene 478667 DogEntrez Gene 100426721 MonkeyEntrez Gene 5984 Human
  UniProt ID P35249
• Background: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
• Synonyms: A1; RFC37

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Mismatch repair, Nucleotide excision repair