KIF25 Mouse Monoclonal Antibody [Clone ID: OTI1H1]

CAT#: CF505427

Carrier-free (BSA/glycerol-free) KIF25 mouse monoclonal antibody, clone OTI1H1 (formerly 1H1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIF25 (RC217730, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF25. Positive lysates LY417362 (100ug) and LC417362 (20ug) can be purchased separately from OriGene.

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  Anti-KIF25 mouse monoclonal antibody (TA505427) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KIF25 (RC217730).

Specifications

Product Data

• Clone Name: OTI1H1
• Applications: IF, WB
• Recommended Dilution: WB 1:1000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KIF25(NP_005346) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.1 kDa
• Gene Name: kinesin family member 25
• Database Link:
  NP_005346
  Entrez Gene 3834 Human
  UniProt ID Q9UIL4
• Background: The protein encoded by this gene is a member of the kinesin-like protein family. Protein family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. However, the particular function of this gene product has not yet been determined. Two alternatively spliced transcript variants which encode products have been described. Other splice variants have been found that lack exon 2 and the initiation codon for translation. [provided by RefSeq, Jul 2008]
• Synonyms: KNSL3

Reference Data

• Protein Families: Druggable Genome