PALS1 Mouse Monoclonal Antibody [Clone ID: OTI2D3]

CAT#: CF504451

Carrier-free (BSA/glycerol-free) MPP5 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MPP5 (Cat# RC224752, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MPP5(Cat# TA504451). Positive lysates LY411659 (100ug) and LC411659 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MPP5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Flow cytometric Analysis of Hela cells, using anti-MPP5 antibody (TA504451), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-MPP5 antibody (TA504451), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI2D3
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Dog, Rat, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MPP5(NP_071919) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77.1 kDa
• Gene Name: protein associated with LIN7 1, MAGUK family member
• Database Link:
  NP_071919
  Entrez Gene 314259 RatEntrez Gene 490740 DogEntrez Gene 64398 Human
  UniProt ID Q8N3R9
• Background: This gene encodes a member of the p55-like subfamily of the membrane-associated guanylate kinase (MAGUK) gene superfamily. The encoded protein participates in the polarization of differentiating cells, has been shown to regulate myelinating Schwann cells (PMID: 20237282), and is one of the components of the Crumbs complex in the retina. Mice which express lower levels of the orthologous protein have retinal degeneration and impaired vision (PMID: 22114289). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
• Synonyms: PALS1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Tight junction