CARKL (SHPK) Mouse Monoclonal Antibody [Clone ID: OTI1D2]

CAT#: CF503209

Carrier-free (BSA/glycerol-free) SHPK mouse monoclonal antibody, clone OTI1D2 (formerly 1D2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPK (RC204421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPK. Positive lysates LY415695 (100ug) and LC415695 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SHPK monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Anti-SHPK mouse monoclonal antibody (TA503209) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHPK (RC204421).

Specifications

Product Data

• Clone Name: OTI1D2
• Applications: IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SHPK(NP_037408) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.3 kDa
• Gene Name: sedoheptulokinase
• Database Link:
  NP_037408
  Entrez Gene 74637 MouseEntrez Gene 287479 RatEntrez Gene 23729 Human
  UniProt ID Q9UHJ6
• Background: The protein encoded by this gene has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements. [provided by RefSeq, Jul 2008]
• Synonyms: CARKL; SHK

Reference Data

• Protein Families: Druggable Genome