PDHA1 Mouse Monoclonal Antibody [Clone ID: OTI2B10]

CAT#: CF502704

Carrier-free (BSA/glycerol-free) PDHA1 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDHA1 (RC201831, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDHA1. Positive lysates LY400110 (100ug) and LC400110 (20ug) can be purchased separately from OriGene.

• 

  Anti-PDHA1 mouse monoclonal antibody (TA502704) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDHA1 (RC201831).

• 

  HEK293T cells transfected with either RC201831 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDHA1 antibody (TA502704), and then analyzed by flow cytometry.

• 

  Flow cytometric Analysis of Hela cells, using anti-PDHA1 antibody (TA502704), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI2B10
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDHA1 (NP_000275) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.2 kDa
• Gene Name: pyruvate dehydrogenase E1 subunit alpha 1
• Database Link:
  NP_000275
  Entrez Gene 18597 MouseEntrez Gene 29554 RatEntrez Gene 5160 Human
  UniProt ID P08559
• Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Synonyms: PDHA; PDHAD; PDHCE1A; PHE1A

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine biosynthesis