RABL2A Mouse Monoclonal Antibody [Clone ID: OTI4A8]

CAT#: CF502018

Carrier-free (BSA/glycerol-free) RABL2A mouse monoclonal antibody, clone OTI4A8 (formerly 4A8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RABL2A (RC211854, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RABL2A. Positive lysates LY416219 (100ug) and LC416219 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RABL2A monoclonal antibody.

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  Anti-RABL2A mouse monoclonal antibody (TA502018) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RABL2A (RC211854).

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  HEK293T cells transfected with either RC211854 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RABL2A antibody (TA502018), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-RABL2A antibody (TA502018), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI4A8
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RABL2A (NP_009013) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.9 kDa
• Gene Name: RAB, member of RAS oncogene family like 2A
• Database Link:
  NP_009013
  Entrez Gene 362987 RatEntrez Gene 11159 Human
  UniProt ID Q9UBK7
• Background: This gene is a member of the RAB gene family which belongs to the RAS GTPase superfamily. The proteins in the family of RAS-related signaling molecules are small GTP-binding proteins that play important roles in the regulation of exocytotic and endocytotic pathways. This gene maps to the site of an ancestral telomere fusion event and may be a subtelomeric gene. Two alternatively spliced transcript variants have been identified, both encoding the same protein. [provided by RefSeq]
• Synonyms: FLJ78724; MGC117180

Reference Data

• Protein Families: Druggable Genome